Calabrese Lab Projects
Electrophysiology of HN cells
Network model of the leech heartbeat timing network
Neuronal networks that produce rhythmic
motor patterns that underlie behaviors such as swimming in the lamprey or
the beating of crayfish swimmerets can be described as chains of coupled
segmental oscillators. In these systems, progressive phase differences
between segments are essential for the generation of appropriate motor
output. The neuronal network that paces the heartbeat of the leech can also
be viewed as a chain of coupled oscillators, albeit with a length of only
two segments. In the leech there are two centers of oscillation in the 3rd
and 4th ganglion. These centers are coupled by coordinating fibers which
are the neuritic processes of heart interneurons of the 1st and 2nd ganglia. Together these
neurons form the timing network that paces the heartbeat of the leech. We
are studying the intersegmental coordination of
segmental oscillators in the leech using a realistic neuronal network
model. Individual neurons are modeled as single compartments with Hodgkin
and Huxley type conductances. We are testing the
idea that the observed 15% phase lag between the segmental oscillators (the
4th ganglion leads the 3rd) may be the result of inherent differences in
the periods of the segmental oscillators. We are also exploring the
possibility that asymmetries in the synaptic coupling between the
oscillators may affect their phase relationship.
Calcium imaging and fluorescent study of HN cells
How does intracellular Ca concentration
correlate to spiking activity of HN cell? What is its contribution into
synaptic transmission? Does intracellular Ca regulate Ca channels and other
ion channels in HN cells? Which mechanisms regulate Ca homeostasis/turnover
in HN cells? What is the spatial distribution of Ca channels and stores in
the HN neuron? Where in the neuron are Ca input
and release initiated? Is there any relation and coordination between
extracellular Ca input and release of Ca from intracellular stores? What is
the spatial distribution of the inhibitory synaptic connection between HN
neurons? Is the inhibitory synaptic connection monosynaptic or multisynaptic? Is there a critical quantity of synapses
which should be switched on to maintain the reciprocal interaction of HN
cells? To answer these questions, we are observing HN cells filled with
calcium-sensitive dyes, and are studying effects of destroying small parts
of HN cells with focused light.
Anatomically realistic Modeling
How does the shape of an HN neuron
contribute to its behavior? How do different spatial distributions of ion
channels affect the cell's computation? Are these anatomical contributions
important to the HN network as a whole? To address these questions, we are
building anatomically realistic, multi-compartmental models of HN cells.
Half-Center Oscillator
model database (HCO-db)
Last updated June
7, 2012. Please send comments to adolocm@emory.edu.
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