E. coli Electroporation
Note: Dialysis helps you to get more colonies (30 min)
1) Get electrocompetant E. coli (DH5a) from the -80C freezer and thaw on ice.
2) Cool cuvette on ice.
3) Add 40 ul E.coli to cuvette. Add 1-10 ul DNA (50 ng-500 ng). Mix by thumping on table. Do a control with no DNA.
4) Remove cuvette from ice. Dry all sides and bottom with a KimWipe.
5) Place in electroporation chamber. Apply voltage is 1800v.
6) Pulse electricity until it beeps. Place back on ice.
7) Add 1 ml LB and incubate at 37C for 30 min.
8) Spin down. Pour off sup leaving ~100ul. Resuspend.
9) Plate entire suspension on appropriate pre-warmed LB plate.
10) Grow at 37C.
