X-gal Plates (500ml)
1) Autoclave separately on liquid setting for 40 minutes:
In a 200ml flask
3.35g Bacto Yeast Nitro Base without Amino Acids
1g Dropout Mix
50ml dH2O
In a 1L flask
10g Bacto Agar
stir bar
350ml dH2O
In a 200ml flask
3.5g dibasic sodium phosphate
1.5g monobasic sodium phosphate
50ml dH2O
2) Cool down
3) Mix
4) Add sugar (50ml of glucose or 50ml of galactose)
5) Add X-gal (400ul of 100mg X-gal/1ml N,N-dimethyl formamide or 800ul of 50mg/ml)
6) Pour
7) Wrap in foil
